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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Laboratory Operations | 5-10% | - Laboratory mathematics and instrumentation - Pre-analytical, analytical, and post-analytical processes - Quality control and quality assurance - Laboratory safety and regulatory compliance |
| Topic 2: Urinalysis and Other Body Fluids | 5-10% | - Cerebrospinal, serous, and synovial fluids - Semen and amniotic fluid testing - Physical, chemical, and microscopic analysis - Urine formation and composition |
| Topic 3: Microbiology | 15-20% | - Specimen collection and culture techniques - Bacteriology and antimicrobial susceptibility - Identification of pathogenic microorganisms - Mycology, parasitology, and virology |
| Topic 4: Immunology and Serology | 5-10% | - Immune system principles and components - Infectious disease serology - Immunologic testing methodologies - Autoimmune and immunodeficiency disorders |
| Topic 5: Hematology | 20-25% | - White blood cell morphology and diseases - Red blood cell physiology and disorders - Routine and automated hematology procedures - Hemostasis and coagulation testing |
| Topic 6: Clinical Chemistry | 20-25% | - Electrolytes, acid-base balance, and blood gases - Endocrinology and therapeutic drug monitoring - Toxicology and liver/kidney function tests - Carbohydrates, lipids, proteins, and enzymes |
| Topic 7: Blood Banking (Immunohematology) | 15-20% | - Transfusion reactions and adverse events - Compatibility testing - Blood components and storage - Blood group systems and antigens - Antibody detection and identification |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. Standard precautions should be followed:
A) when a patient is known to have hepatitis B
B) if a patient is in isolation
C) when a patient is known to be HIV positive
D) with all patients, at all times
2. The disc diffusion method for antibiotic susceptibility testing is the Kirby-Bauer method. The agar used for this procedure is Meuller-Hinton.
Which of the following media is commonly used when performing the Kirby-Bauer disk diffusion?
A) Mueller-Hinton agar
B) MacConkey agar
C) Thayer-Martin agar
D) Chocolate agar
3. Fibers can be mistaken for casts on occasion, especially hyaline casts. Fibers, however, are usually thinner and appear less uniform in structure than casts do.
Urinalysis & Other Body Fluids
Which of the following artifacts may be mistaken for casts?
A) Coverslip scratches
B) Fibers
C) Air bubbles
D) Talc particles
4. Provide the equivalent measurement for one gallon.
Question options:
A) 3.79 liters
B) 1 liter
C) 2.5 liters
D) 4.2 liters
5. Though automated extraction machines have many benefits over manual methods the costs are high and generally require a high throughput of samples in order to justify the costs.
Automated extraction has many benefits over the traditional manual methods. The most important benefit is that the nucleic acid isolated is constantly consistent. There is a reduced amount of manipulation with dramatically decreases the chance of cross contamination. Also, automated extraction machines are considered moderate complexity and can be performed by a wider variety of laboratory professionals.
All of the following are considered benefits of automated isolation and extraction equipment EXCEPT:
A) Automated isolation equipment requires less manipulation
B) Automated isolation equipment is cheaper and more practical
C) Automated isolation equipment is considered moderate complexity
D) Automated isolation equipment provides consistent results
Solutions:
| Question # 1 Answer: D | Question # 2 Answer: A | Question # 3 Answer: B | Question # 4 Answer: A | Question # 5 Answer: B |
